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immunization grade bovine type ii collagen, lyophilized  (Chondrex Inc)


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    Chondrex Inc immunization grade bovine type ii collagen, lyophilized
    Immunization Grade Bovine Type Ii Collagen, Lyophilized, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 96/100, based on 1574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+ii+bovine/Immunization+Grade+Bovine+Type+II+Collagen%2C+lyophilized/custom%4020021%4042573912
    Average 96 stars, based on 1574 article reviews
    immunization grade bovine type ii collagen, lyophilized - by Bioz Stars, 2026-10
    96/100 stars

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    Adjuvant:

    Article Title: Fluorinated polyamidoamine dendrimer-mediated miR-23b delivery for the treatment of experimental rheumatoid arthritis in rats
    Article Snippet: TRNzol Universal reagent (DP424) for RNA isolation and Fast qPCR Master Mix (High Rox) (B639273) were purchased from Tiangen Biotech (Beijing, China) and Sangon Biotech (Shanghai, China), respectively. .. The complete Freund’s adjuvant (CFA) (7009 and 7027), incomplete Freund’s adjuvant (IFA) (7002) and type II bovine (20022) were purchased from Chondrex (Woodinville, WA). .. The following kits were used in these studies: one-step TUNEL apoptosis assay kit (C1086), Beyotime (Nanjing, China); Annexin V-FITC/PI apoptosis detection kit (BB4101) and BCA protein assay kit (BB3401), Bestbio (Shanghai, China); caspase-3 (G8090), −8 (G8200) and −9 (G8210) activity assay kits, Promega (Madison, WI); PrimeScript RT reagent kit (RR037A) and TB Green Premix Ex Taq (Tli RNaseH Plus) kit (RR820A), Takara (Dalian, China); rat TNF ELISA kit (RTA00), rat IL1B ELISA kit (RLB00), rat IL6 ELISA kit (R6000B), mouse TNF ELISA kit (MTA00B), mouse IL1B ELISA kit (MLB00C) and mouse IL6 ELISA kit (M6000B), R&D (Minneapolis, MN).

    Article Title: Near-infrared light-driven metabolic reprogramming of synoviocytes for the treatment of rheumatoid arthritis
    Article Snippet: TRNzol Universal reagent (DP424) and Fast qPCR Master Mix (High Rox) (B639273) were purchased from TIANGEN Biotech (Beijing, China) and Sangon Biotech (Shanghai, China), respectively. .. The complete Freund’s adjuvant (CFA), incomplete Freund’s adjuvant (IFA) and type II bovine were purchased from Chondrex (WA, USA). .. The following kits were used in these studies: BCA protein assay kit (BB3401) was purchased from SparkJade (Shandong, China); PrimeScript RT reagent kit (RR037A) and TB Green Premix Ex Taq (Tli RNaseH Plus) kit (RR820A) were acquired from TaKaRa (Dalian, China); Glucose Assay Kit with O-toluidin (S0201S), Reactive Oxygen Species Assay Kit (S0033S) and Mitochondrial membrane potential assay kit with JC-1 (C2006) were purchased form Beyotime (Jiangsu, China); L-Lactate assay kit (ab65330) was purchased from Abcam (Shanghai, China); CellTiter-Glo ® Luminescent Cell Viability Assay (CTG) was purchased from Promega (WI, USA).

    Article Title: DNAzyme targeting RIP3 suppresses NLRP3-mediated necroinflammation for the treatment of inflammatory diseases
    Article Snippet: Dihydroethidium (DHE, D0079) was acquired from MCE (Monmouth Junction, NJ, USA). .. The reagents used in the construction of animal models were listed as follows: concanavalin A (Con A) and uric acid (U0881), Sigma–Aldrich (St. Louis, MO, USA); complete Freund’s adjuvant (CFA) (7009), incomplete Freund’s adjuvant (IFA) (7002) and type II bovine (20022), Chondrex (Woodinville, WA). .. The relevant kits were used in the present studies: CellTiter-Glo Luminescent Cell Viability Assay kit, Promega (Madison, WI, USA); Duolink In-Situ Red Starter kit, Mouse/Rabbit (DUO92101), Sigma–Aldrich (St. Louis, MO, USA); IL6 Mouse ELISA kit (BMS603-2), IL1 β Mouse ELISA kit (BMS6002) and TNF Mouse ELISA kit (BMS607-3), Invitrogen (Carlsbad, CA, USA); IL18 Mouse ELISA kit (MM-0169M2) and HMGB1 Mouse ELISA kit (MM-44107M1), Meimian Industrial Co., Ltd. (Jiangsu, China); PrimeScript RT reagent kit (RR037A) and TB Green Premix Ex Taq kit (Tli RNaseH Plus, RR820A), TaKaRa (Dalian, China); BCA protein assay kit (BB3401), Bestbio (Shanghai, China); DCF-DA Cellular Reactive Oxygen Species Detection kit (S0033S), Beyotime (Nanjing, China); Fluorescent Multiplex Reagent kit (RC0086-34RM), Recordbio (Shanghai, China); Safranin-O/Fast Green kit (G1053), Toluidine blue dye solution (G1032) and H&E Staining kit (G1003), Servicebio (Wuhan, China).

    Article Title: Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner
    Article Snippet: On day 0, each DBA/1 mouse was immunized by intradermal injection of 200 μg type II bovine collagen (#20021, Chondrex) emulsified in Freund’s complete adjuvant (#7009, Chondrex) at the base of tail and type II chicken collagen was used for the immunization of C57BL/6 mice. .. On day 21, the mice were given a booster immunization with 200 μg type II bovine or chicken collagen emulsified in Freund’s incomplete adjuvant (#7002, Chondrex). .. Neutralizing anti-KIAA1199 mAb was obtained by genetic immunization method developed previously , , 200 μg neutralizing anti-KIAA1199 mAb or purified mouse IgG (Sigma-Aldrich) were intraperitoneally injected into two groups of CIA mice ( n = 10 per group) constructed with DBA/1 weekly, and a total of four injections from day 22 to day 43.

    Article Title: Fluorinated polyamidoamine dendrimer-mediated miR-23b delivery for the treatment of experimental rheumatoid arthritis in rats.
    Article Snippet: TRNzol Universal reagent (DP424) for RNA isolation and Fast qPCR Master Mix (High Rox) (B639273) were purchased from Tiangen Biotech (Beijing, China) and Sangon Biotech (Shanghai, China), respectively. .. The complete Freund’s adjuvant (CFA) (7009 and 7027), incomplete Freund’s adjuvant (IFA) (7002) and type II bovine (20022) were purchased from Chondrex (Woodinville, WA). .. The following kits were used in these studies: one-step TUNEL apoptosis assay kit (C1086), Beyotime (Nanjing, China); Annexin V-FITC/PI apoptosis detection kit (BB4101) and BCA protein assay kit (BB3401), Bestbio (Shanghai, China); caspase-3 (G8090), −8 (G8200) and −9 (G8210) activity assay kits, Promega (Madison, WI); PrimeScript RT reagent kit (RR037A) and TB Green Premix Ex Taq (Tli RNaseH Plus) kit (RR820A), Takara (Dalian, China); rat TNF ELISA kit (RTA00), rat IL1B ELISA kit (RLB00), rat IL6 ELISA kit (R6000B), mouse TNF ELISA kit (MTA00B), mouse IL1B ELISA kit (MLB00C) and mouse IL6 ELISA kit (M6000B), R&D (Minneapolis, MN).

    Immunofluorescence:

    Article Title: Fluorinated polyamidoamine dendrimer-mediated miR-23b delivery for the treatment of experimental rheumatoid arthritis in rats
    Article Snippet: TRNzol Universal reagent (DP424) for RNA isolation and Fast qPCR Master Mix (High Rox) (B639273) were purchased from Tiangen Biotech (Beijing, China) and Sangon Biotech (Shanghai, China), respectively. .. The complete Freund’s adjuvant (CFA) (7009 and 7027), incomplete Freund’s adjuvant (IFA) (7002) and type II bovine (20022) were purchased from Chondrex (Woodinville, WA). .. The following kits were used in these studies: one-step TUNEL apoptosis assay kit (C1086), Beyotime (Nanjing, China); Annexin V-FITC/PI apoptosis detection kit (BB4101) and BCA protein assay kit (BB3401), Bestbio (Shanghai, China); caspase-3 (G8090), −8 (G8200) and −9 (G8210) activity assay kits, Promega (Madison, WI); PrimeScript RT reagent kit (RR037A) and TB Green Premix Ex Taq (Tli RNaseH Plus) kit (RR820A), Takara (Dalian, China); rat TNF ELISA kit (RTA00), rat IL1B ELISA kit (RLB00), rat IL6 ELISA kit (R6000B), mouse TNF ELISA kit (MTA00B), mouse IL1B ELISA kit (MLB00C) and mouse IL6 ELISA kit (M6000B), R&D (Minneapolis, MN).

    Article Title: Near-infrared light-driven metabolic reprogramming of synoviocytes for the treatment of rheumatoid arthritis
    Article Snippet: TRNzol Universal reagent (DP424) and Fast qPCR Master Mix (High Rox) (B639273) were purchased from TIANGEN Biotech (Beijing, China) and Sangon Biotech (Shanghai, China), respectively. .. The complete Freund’s adjuvant (CFA), incomplete Freund’s adjuvant (IFA) and type II bovine were purchased from Chondrex (WA, USA). .. The following kits were used in these studies: BCA protein assay kit (BB3401) was purchased from SparkJade (Shandong, China); PrimeScript RT reagent kit (RR037A) and TB Green Premix Ex Taq (Tli RNaseH Plus) kit (RR820A) were acquired from TaKaRa (Dalian, China); Glucose Assay Kit with O-toluidin (S0201S), Reactive Oxygen Species Assay Kit (S0033S) and Mitochondrial membrane potential assay kit with JC-1 (C2006) were purchased form Beyotime (Jiangsu, China); L-Lactate assay kit (ab65330) was purchased from Abcam (Shanghai, China); CellTiter-Glo ® Luminescent Cell Viability Assay (CTG) was purchased from Promega (WI, USA).

    Article Title: DNAzyme targeting RIP3 suppresses NLRP3-mediated necroinflammation for the treatment of inflammatory diseases
    Article Snippet: Dihydroethidium (DHE, D0079) was acquired from MCE (Monmouth Junction, NJ, USA). .. The reagents used in the construction of animal models were listed as follows: concanavalin A (Con A) and uric acid (U0881), Sigma–Aldrich (St. Louis, MO, USA); complete Freund’s adjuvant (CFA) (7009), incomplete Freund’s adjuvant (IFA) (7002) and type II bovine (20022), Chondrex (Woodinville, WA). .. The relevant kits were used in the present studies: CellTiter-Glo Luminescent Cell Viability Assay kit, Promega (Madison, WI, USA); Duolink In-Situ Red Starter kit, Mouse/Rabbit (DUO92101), Sigma–Aldrich (St. Louis, MO, USA); IL6 Mouse ELISA kit (BMS603-2), IL1 β Mouse ELISA kit (BMS6002) and TNF Mouse ELISA kit (BMS607-3), Invitrogen (Carlsbad, CA, USA); IL18 Mouse ELISA kit (MM-0169M2) and HMGB1 Mouse ELISA kit (MM-44107M1), Meimian Industrial Co., Ltd. (Jiangsu, China); PrimeScript RT reagent kit (RR037A) and TB Green Premix Ex Taq kit (Tli RNaseH Plus, RR820A), TaKaRa (Dalian, China); BCA protein assay kit (BB3401), Bestbio (Shanghai, China); DCF-DA Cellular Reactive Oxygen Species Detection kit (S0033S), Beyotime (Nanjing, China); Fluorescent Multiplex Reagent kit (RC0086-34RM), Recordbio (Shanghai, China); Safranin-O/Fast Green kit (G1053), Toluidine blue dye solution (G1032) and H&E Staining kit (G1003), Servicebio (Wuhan, China).

    Article Title: Fluorinated polyamidoamine dendrimer-mediated miR-23b delivery for the treatment of experimental rheumatoid arthritis in rats.
    Article Snippet: TRNzol Universal reagent (DP424) for RNA isolation and Fast qPCR Master Mix (High Rox) (B639273) were purchased from Tiangen Biotech (Beijing, China) and Sangon Biotech (Shanghai, China), respectively. .. The complete Freund’s adjuvant (CFA) (7009 and 7027), incomplete Freund’s adjuvant (IFA) (7002) and type II bovine (20022) were purchased from Chondrex (Woodinville, WA). .. The following kits were used in these studies: one-step TUNEL apoptosis assay kit (C1086), Beyotime (Nanjing, China); Annexin V-FITC/PI apoptosis detection kit (BB4101) and BCA protein assay kit (BB3401), Bestbio (Shanghai, China); caspase-3 (G8090), −8 (G8200) and −9 (G8210) activity assay kits, Promega (Madison, WI); PrimeScript RT reagent kit (RR037A) and TB Green Premix Ex Taq (Tli RNaseH Plus) kit (RR820A), Takara (Dalian, China); rat TNF ELISA kit (RTA00), rat IL1B ELISA kit (RLB00), rat IL6 ELISA kit (R6000B), mouse TNF ELISA kit (MTA00B), mouse IL1B ELISA kit (MLB00C) and mouse IL6 ELISA kit (M6000B), R&D (Minneapolis, MN).



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    Phenotypic Characterization of Collagen Induced Arthritis in DBA/1J Mice. DBA/1J mice were immunized with bovine type II collagen (N = 11–55; arthritic: n = 6–18; non-arthritic: n = 5–37) or PBS (N = 4–14). A) Representative photographs and E) 3D-rendered micro-CT radiographs of arthritic (red arrow = bone erosion), non-arthritic, and PBS-injected mouse hind limbs. Changes in B) clinical arthritis score, C) anti-bovine type II collagen (CII) IgG and D) anti-mouse CII IgG over time. Dashed line represents the antibody positivity cut-off, indicating the limit of detection. F) Hind limb bone mineral density and G) bone mineral density of bCII-immunized fore and hind limbs over time. H) Pain-like behaviour at day 47 post primary immunization. Graphs show the median [IQR], with each symbol in F-H) representing an individual mouse. For B-D), statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test, with resulting p-values shown in the graphs. Kruskal-Wallis with Dunn's multiple comparisons test was used for F) and H), and the resulting p-values were p = 0.0008 and p = 0.0003 respectively, with p-values for pairwise comparisons indicated on the graphs. For G), a Spearman correlation coefficient (r) was calculated and p < 0.05 was considered significant.

    Journal: Journal of Translational Autoimmunity

    Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

    doi: 10.1016/j.jtauto.2025.100345

    Figure Lengend Snippet: Phenotypic Characterization of Collagen Induced Arthritis in DBA/1J Mice. DBA/1J mice were immunized with bovine type II collagen (N = 11–55; arthritic: n = 6–18; non-arthritic: n = 5–37) or PBS (N = 4–14). A) Representative photographs and E) 3D-rendered micro-CT radiographs of arthritic (red arrow = bone erosion), non-arthritic, and PBS-injected mouse hind limbs. Changes in B) clinical arthritis score, C) anti-bovine type II collagen (CII) IgG and D) anti-mouse CII IgG over time. Dashed line represents the antibody positivity cut-off, indicating the limit of detection. F) Hind limb bone mineral density and G) bone mineral density of bCII-immunized fore and hind limbs over time. H) Pain-like behaviour at day 47 post primary immunization. Graphs show the median [IQR], with each symbol in F-H) representing an individual mouse. For B-D), statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test, with resulting p-values shown in the graphs. Kruskal-Wallis with Dunn's multiple comparisons test was used for F) and H), and the resulting p-values were p = 0.0008 and p = 0.0003 respectively, with p-values for pairwise comparisons indicated on the graphs. For G), a Spearman correlation coefficient (r) was calculated and p < 0.05 was considered significant.

    Article Snippet: Immunization-grade bovine type II collagen (bCII) protein was purchased from Chondrex Inc (20022; USA).

    Techniques: Micro-CT, Injection

    Splenic T Cell Proliferation Following Stimulation with Type II Collagen Protein. Splenocytes isolated at day 49 post primary immunization from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 8; non-arthritic: N = 7) or PBS (N = 10) were cultured in media alone or 80 μg/mL bCII protein. Following 72 h incubation, A) CD4 + and B) CD8 + T cell proliferation following stimulation with bCII was measured by flow cytometry and is shown as a mean (±SD) stimulation index, with data points representing individual mice. Stimulation indices greater than 1.6 (dashed line) were considered a positive response. Statistical analysis was performed using one-way ANOVA with Tukey's multiple comparisons test, and the resulting p-values were p = 0.0054 and p = 0.0987 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

    Journal: Journal of Translational Autoimmunity

    Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

    doi: 10.1016/j.jtauto.2025.100345

    Figure Lengend Snippet: Splenic T Cell Proliferation Following Stimulation with Type II Collagen Protein. Splenocytes isolated at day 49 post primary immunization from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 8; non-arthritic: N = 7) or PBS (N = 10) were cultured in media alone or 80 μg/mL bCII protein. Following 72 h incubation, A) CD4 + and B) CD8 + T cell proliferation following stimulation with bCII was measured by flow cytometry and is shown as a mean (±SD) stimulation index, with data points representing individual mice. Stimulation indices greater than 1.6 (dashed line) were considered a positive response. Statistical analysis was performed using one-way ANOVA with Tukey's multiple comparisons test, and the resulting p-values were p = 0.0054 and p = 0.0987 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

    Article Snippet: Immunization-grade bovine type II collagen (bCII) protein was purchased from Chondrex Inc (20022; USA).

    Techniques: Isolation, Cell Culture, Incubation, Flow Cytometry

    RA-Specific Serum IgG Antibodies in DBA/1J Mice. Sera were collected biweekly from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 9–18; non-arthritic: N = 17–37), or PBS (N = 14) and tested via indirect ELISA for A) anti-CitP, and B) anti-HomoCitP IgG antibodies. Graphs show the median [IQR] for each group, with a cut-off indicating the limit of detection (dashed line). Statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. The resulting p-values are indicated on the graphs.

    Journal: Journal of Translational Autoimmunity

    Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

    doi: 10.1016/j.jtauto.2025.100345

    Figure Lengend Snippet: RA-Specific Serum IgG Antibodies in DBA/1J Mice. Sera were collected biweekly from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 9–18; non-arthritic: N = 17–37), or PBS (N = 14) and tested via indirect ELISA for A) anti-CitP, and B) anti-HomoCitP IgG antibodies. Graphs show the median [IQR] for each group, with a cut-off indicating the limit of detection (dashed line). Statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. The resulting p-values are indicated on the graphs.

    Article Snippet: Immunization-grade bovine type II collagen (bCII) protein was purchased from Chondrex Inc (20022; USA).

    Techniques: Indirect ELISA